Attachment, Adjustment and Anxiety in Women Pregnant with In Vitro Fertilization: A Comparison with the Control Group
Keywords:
In Vitro Fertilization (IVF), Maternal, Fetal Attachment, Pregnancy Anxiety, Prenatal Self, Evaluation, PregnancyAbstract
Introduction and Aim: Pregnancy achieved through in vitro fertilization (IVF) may be associated with distinct psychological experiences because of previous infertility, prolonged treatment, uncertainty regarding treatment outcomes, and concerns about pregnancy maintenance. This study aimed to compare maternal-fetal attachment, prenatal maternal adjustment, and pregnancy anxiety between women who conceived through IVF and women with natural pregnancies.
Methodology: This applied study employed a descriptive causal-comparative (ex post facto) design. The study population comprised pregnant women attending the Thamar Infertility and Recurrent Miscarriage Clinic in Shiraz, Iran, during June 2025. Using matched purposive sampling, 60 pregnant women were recruited and allocated to two independent groups of 30 women with IVF pregnancies and 30 women with natural pregnancies. The groups were matched on major demographic and background characteristics. Data were collected using Cranley’s Maternal-Fetal Attachment Scale, Lederman’s Prenatal Self-Evaluation Questionnaire, and Vandenberg’s Pregnancy-Related Anxiety Questionnaire. Chi-square, Fisher’s exact test, one-way analysis of variance (ANOVA), and multivariate analysis of variance (MANOVA) were performed using SPSS version 27, with statistical significance set at p<0.05.
Findings: MANOVA demonstrated a significant overall difference between the two groups across the combined dependent variables (Wilks’ λ=0.57, F(3,56)=13.92, p<0.001, Partial η²=0.43). Subsequent univariate analyses indicated that women with IVF pregnancies had significantly greater maternal-fetal attachment than women with natural pregnancies (F(1,58)=28.45, p<0.001, Partial η²=0.33). Prenatal adjustment was also significantly higher in the IVF group (F(1,58)=5.12, p=0.027, Partial η²=0.08). In addition, women who conceived through IVF experienced significantly greater pregnancy anxiety than those with natural pregnancies (F(1,58)=10.28, p=0.002, Partial η²=0.15).
Conclusion: IVF pregnancy appears to be characterized by a dual psychological pattern in which stronger maternal-fetal attachment and greater prenatal adjustment coexist with elevated pregnancy anxiety. Integrating routine psychological assessment with targeted counseling and supportive interventions into prenatal care may therefore be particularly important for women who conceive through assisted reproductive technologies.
Downloads
References
Abasi, A., & Tafazzoli, M. (2009). The Effect of Attachment Behavior Training on Maternal-Fetal Attachment in Primigravida Mothers. Avicenna Journal of Nursing and Midwifery, 17(12), 35-45. https://doi.org/10.12968/bjom.1998.6.3.188
Abasi, A., Tafazzoli, M., & Esmaili, H. (2010). The Effect of Maternal Fetal Movement Counting on Maternal-Fetal Attachment in Primigravida Mothers. Journal of Mazandaran University of Medical Sciences, 20(77), 53-60. https://doi.org/10.3906/sag-1204-97
Alizadeh, T. (2015). The Relationship between Self-Esteem, Locus of Control, Stress, and Infertility in Infertile Men and Women in Tehran Tarbiat Moallem University].
Bodecs, T., Horvath, B., Szilagyi, E., Gonda, X., Rihmer, Z., & Sandor, J. (2011). Effects of Depression, Anxiety, Self-Esteem, and Health Behaviour on Neonatal Outcomes in a Population-Based Hungarian Sample. European Journal of Obstetrics & Gynecology and Reproductive Biology, 154(1), 45-50. https://doi.org/10.1016/j.ejogrb.2010.08.021
Delpasand, T., Ahadi, H., & Jomhari, F. (2022). The Effectiveness of Stress Management Training (Cognitive-Behavioral Strategies) on Perceived Stress and In Vitro Fertilization Outcomes in Infertile Women. Journal of Mashhad University of Medical Sciences, 65(4), 1975-1983.
Heredia, A., Padilla, F., Castilla, J. A., & Garcia-Retamero, R. (2020). Effectiveness of a Psychological Intervention Focused on Stress Management for Women Prior to IVF. Journal of Reproductive and Infant Psychology, 38(2), 113-126. https://doi.org/10.1080/02646838.2019.1601170
Holley, S. R., Pasch, L. A., & Domar, A. D. (2021). Dealing with the Emotional Distress Following Failed IVF. In Assisted Reproduction Techniques: Challenges and Management Options (pp. 678-683). https://doi.org/10.1002/9781119622215.ch107
Hui Choi, W., Lee, G., Chan, C. H., Cheung, R. Y., Lee, L. Y., & Chan, C. (2012). The Relationships of Social Support. Journal of Advanced Nursing, 68(12), 2633-2645.
Karamoozian, M., Askarizadeh, G., & Behrouz, N. (2016). Investigating the Psychometric Properties of the Pregnancy Anxiety Questionnaire. Journal of Clinical Nursing and Midwifery, 5(4), 22-34. https://doi.org/10.4103/ijnmr.ijnmr_324_21
Kornas-Biela, D., & Walczak-Kozłowska, T. (2022). Maternal Adult Attachment and Maternal–Fetal Attachment in the Context of Romantic Relationship Quality. Frontiers in Psychiatry, 13, 935871. https://doi.org/10.3389/fpsyt.2022.935871
Liu, H., Huang, F., Gao, Y., Wang, M., Lin, Q., Kong, Y., Zhou, R., Zhang, C., & Chen, Y. (2025). Correction: Network Analysis of Depression and Anxiety Symptoms and Their Associations with Cognitive Fusion Among Pregnant Women. BMC psychiatry, 25(1), 771. https://doi.org/10.1186/s12888-025-07204-5
Mazzeschi, C., Pazzagli, C., Radi, G., Raspa, V., & Buratta. (2015). Antecedents of Maternal Parenting Stress: The Role of Attachment Style, Prenatal Attachment, and Dyadic Adjustment in First-Time Mothers. Frontiers in psychology, 6, 1443. https://doi.org/10.3389/fpsyg.2015.01443
McMahon, C. A., & Ungerer, J. A. (2014). Anxiety during Pregnancy and Fetal Attachment after In-Vitro Fertilization Conception. Human Reproduction, 12(1), 176-182. https://doi.org/10.1093/humrep/12.1.176
Moshki, M., Tavakolizadeh, J., Paykani, T., Sebghati-Oghaz, A., & Dehnoalian, A. (2025). The Effect of Social Support Based Intervention on COVID-19 Anxiety in Pregnant Women: A Randomized Pretest-Posttest Control Group Design. https://doi.org/10.21203/rs.3.rs-6653049/v1
Nasrabadi, T., Akhavan Amjadi, M., & Hosseinzadeh, N. (2022). Investigating the Relationship between Pregnancy Anxiety, Sleep Quality, and Gestational Diabetes. Hayat, 28(3), 272-283. https://doi.org/10.47939/mh.v3i4(06).19
Olivius, C., Friden, B., Borg, G., & Bergh, C. (2014). Why Do Couples Discontinue In-Vitro Fertilization Treatment? A Cohort Study. Fertility and sterility, 81(2), 258-261. https://doi.org/10.1016/j.fertnstert.2003.06.029
Pearlstein, T. (2015). Depression during Pregnancy. Best practice & research Clinical obstetrics & gynaecology. https://doi.org/10.1016/j.bpobgyn.2015.04.004
Pellerone, M., Martinez-Torvisco, J., Razza, S. G., Commodari, E., & Miccichè, S. (2023). Precursors of Prenatal Attachment and Anxiety during Pregnancy in Women Who Procreate Naturally and Pregnant Women Following Assisted Reproduction Technology. International journal of environmental research and public health, 20(20), 6945. https://doi.org/10.3390/ijerph20206945
Ranjbar, F., Warmelink, C. J., Mousavi, R., & Gharacheh, M. (2021). Maternal-Fetal Attachment and Anxiety in Pregnant Women Who Conceived through Assisted Reproductive Technology: A Longitudinal Study. International Journal of Reproductive BioMedicine, 19(12), 1075-1084. https://doi.org/10.18502/ijrm.v19i12.10058
Saastad, E., Israel, P., Ahlborg, T., Gunnes, N., & Frøen, J. F. (2019). Fetal Movement Counting—Effects on Maternal-Fetal Attachment: A Multicenter Randomized Controlled Trial. 38(4), 282-293. https://doi.org/10.1111/j.1523-536x.2011.00489.x
Sharif, F., Zare, N., Toosi, M., & Akbarzadeh, M. (2011). The Effect of Learning Attachment Behaviors on Anxiety and Maternal-Fetal Attachment in Primigravida Mothers. Evidence-Based Care, 1(1). https://doi.org/10.1016/j.jnma.2017.03.002
Tafazzoli, M., Asadi, M., Yazdi, S. A. A., & Shakeri, M. T. (2014). The Relationship between Maternal-Fetal Attachment and Exclusive Breastfeeding in Primigravida Women. Journal of Neyshabur University of Medical Sciences, 2(3), 67-75. https://doi.org/10.4103/jdmimsu.jdmimsu_273_20
Vázquez-Lara, M. D., Rúger-Navarrete, A., Mohamed-Abdel-Lah, S., Gómez‐Urquiza, J. L., Fernández-Carrasco, F. J., Díaz, L. R., Caparrós-González, R. A., Palomo-Gómez, R., Riesco-González, F. J., & Vázquez-Lara, J. M. (2025). The Impact of Mindfulness Programmes on Anxiety, Depression and Stress During Pregnancy: A Systematic Review and Meta-Analysis. Healthcare, 13(12), 1378. https://doi.org/10.3390/healthcare13121378
Verhaak, C. M., Smeenk, J. M. J., Evers, A. W. M., Kremer, J. A. M., Kraaimaat, F. W., & Braat, D. D. M. (2007). Women’s Emotional Adjustment to IVF: A Systematic Review of 25 Years of Research. Human Reproduction Update, 13(1), 27-36. https://doi.org/10.1093/humupd/dml040
Yarcheski, A., Mahon, N. E., Yarcheski, T. J., Hanks, M. M., & Cannella, B. L. (2009). A Meta-Analytic Study of Predictors of Maternal-Fetal Attachment. International journal of nursing studies, 46(5), 708-715. https://doi.org/10.1016/j.ijnurstu.2008.10.013
Yeşildere Sağlam, H., Gursoy, E., & Kartal, Y. (2025). The effect of nursing care during pregnancy termination on women's prenatal grief, depression, anxiety, stress and coping levels: A randomized controlled study. Women & Health, 65(1), 50-65. https://doi.org/10.1080/03630242.2024.2441377
Zagami, S. E., Roudsari, R. L., Janghorban, R., Bazaz, S. M. M., Amirian, M., & Allan, H. T. (2019). Infertile Couples' Needs after Unsuccessful Fertility Treatment: A Qualitative Study. Journal of caring sciences, 8(2), 95. https://doi.org/10.15171/jcs.2019.014
Zamani, M., & Seyedmousavi, P. S. (2022). Psychometric Properties of the Persian Version of the Prenatal Self-Evaluation Questionnaire (PSEQ): Assessing Maternal Adjustment during Pregnancy. Payesh, 21(3), 255-265. https://doi.org/10.52547/payesh.21.3.287
Downloads
Publication Timeline
- Submitted
- Revised
- Accepted
Issue
Section
License
Copyright (c) 2025 Zahra Pirhasani (Author); Leila Khabir; Elnaz Fathi Kaljahi (Author)

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.